In the context of the construction of "double first-class" university, facing many problems existing in the biochemical laboratory of our university, it is necessary to increase the opening degree of laboratory in order to play an important role in cultivating innovative talents. According to the current teaching situation of biochemical laboratories, the author put forward the laboratory opening mode based on "basic-improvement" dual-level personnel training and discipline competition. At the same time, in order to ensure the smooth development of laboratory opening, the incentive mechanism of laboratory teachers has been proposed.