Underground media are generally affected by the initial stress of the overlying strata. At the same time, the uncoupled interface is an important influencing factor of seismic response, as well as an essential reservoir environment, reservoir space, and migration channel. It is significant to explore the parameters of the uncoupled interface under stress for identifying oil and gas reservoirs. As an effective means of predicting reservoir physical parameters, pre-stack seismic inversion uses information on uncoupled interface fractures under stress to improve the accuracy of reservoir parameter prediction, overcome the problem of insufficient interface parameter prediction methods, and promote oil and gas reservoir exploration and development. Based on the precise equations for directly characterizing uncoupled interface fracture weakness parameters, this paper uses the ANNI inversion method to realize the nonlinear inversion method of uncoupled interface fracture parameters. The feasibility and practicability of predicting underground medium parameters from seismic response characteristics have been verified through model testing and actual work area application.